In-planta transformation: recent advances
نویسندگان
چکیده
In-planta transformation is an efficient, quick and tissue culture independent system for crop plants improvement. In-planta transformation is a useful system for those plants that lack tissue culture and regeneration system. Therefore, the direct in-planta methods are commonly used for the transformation of many important genes into these plant species. The two most common Agrobacterium mediated in-planta methods such as floral dip and vacuum infiltration have been successfully used by many researchers in both dicot and monocot plants. The main advantages of in-planta transformation are to produce large number of transgenic plants and accumulation of high concentration of total soluble protein in short time. The production of chimera plants and lack of stable transgene expression at T1 or after T1 stage are some of the problems to these methods that need further improvements. The present review mainly focuses on in-planta transformation protocols that were successfully used for many agronomical important crop plants and to describe the various advantages and disadvantages of in-planta transformation methods in different crop species.
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